LOCUS       sequence-117843-         758 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC42
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..106
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(404..423)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     673..691
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 taatccagag gttgattatc gataagcttg atatcgaatt gtacctagtg gaaccggaac
       61 ccttaaacat gtataacttc gtataatgta tgctatacga agttattagg tccctcgacg
      121 aattcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
      181 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg
      241 ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg
      301 tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg
      361 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
      421 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
      481 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      541 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
      601 ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
      661 agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
      721 tcgccctcgc cctcgatctc gaactcgtgg ccgttcac
//
