LOCUS       sequence-117955-         586 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of shRNA targeting human ARF1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA
  TITLE     Isoform-selective effects of the depletion of ADP-ribosylation 
            factors 1-5 on membrane traffic.
  JOURNAL   Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19.
  PUBMED    16030262
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-10"; volume: "16(10)"; pages: "4495-508. Epub 
            2005 Jul 19"
COMMENT     Sequence Label: pSUPER-ARF1b
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(108..124)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(109..125)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(153..171)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(192..208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(205..227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    216..232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(240..246,247..264,265..270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    285..306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(423..440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatttgggaa tcttataagt tctgtatgag accacagatc ccctgacaga gagcgtgtga
       61 acttcaagag agttcacacg ctctctgtca tttttggaaa agcttatcga taccgtcgac
      121 ctcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg
      181 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      481 tcaaaggcgg taatacggtt atccacagaa tcaggagata acgcaggaaa gaacatgtga
      541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgc
//
