LOCUS       sequence-118019-         931 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of hArl3 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL3
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(706..730)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    771..795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(795..814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(796..814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(898..917)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tcaaactcaa tggtgatggt gatgatgacc ggtacgcgta
       61 gaatcgagac cgaggagagg gttagggata ggcttacctt caagcttcga attgatcacc
      121 actttgtaca agaaagctgg tctttcttct ttgcattgac atttttgcag acccagttca
      181 tgccatcctg aacgccctct cctgtgagag ctgagcaaga ctggatctgc cagactcggt
      241 cgcggatggt atgcaggttc agtccttctg caatttcaga ggcaggggct gctgtgagca
      301 aatcctgctt attagcaaag atgagcactg gcacacaact tagtttttct tcctccagta
      361 attccgctag ttcctgaccc gtctcttcaa atctttttct gtctgcactg tcgattacat
      421 atataagaat atcggtattt tcaaaataat tcttccagta tggtctgatt ttcctctgtc
      481 caccaatgtc ccatacattc agtttaaaac cttgtgattg tacacttttg atgttgaaac
      541 cctgtgtagg tgtgatgtgg ctgatgtctt cagatgcaag ctgcttcaga agagtggtct
      601 tgccagcatt atccaagccc aggagaagta ttctcacctc ctggtctggt gcacttttca
      661 acttgcgcaa aattgagagc aagcccatgg ttctatctcc ttctaagcct gcttttttgt
      721 acaaacttgt gatattcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      781 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
      841 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      901 atatcgccga catcaccgat ggggaagatc g
//
