LOCUS       sequence-118140-         902 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of hArl4D with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARL4D-V5-His6
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..97)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(763..787)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    828..852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(852..871)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(853..871)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag
       61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca
      121 ctttgtacaa gaaagctggg tcccgtctct tcttgccacc ccgagctgcc ttcttcctct
      181 tgaggatcat ctcatagagg cgctcaaggc cctgctgcag gcccagaccg tccacagcgc
      241 tgcagccttg cacatgagtg agagtggcgg ctgctagctc tcggactgcc agcctcttct
      301 ccacctcagc agcgctcagt gccccgggct ggtcctgctt gttggccagc accagcactg
      361 gcacgccctg gttgtccgag gcccggctga ttcggtgcaa ctccaccttg gcttcctcca
      421 gccgctcagc ctccgcagcg tccaccacaa acactagacc gtctgtccgg cgggtataag
      481 agcgccacag tggtcgcagc ttctcctgcc ccccgacgtc ccacacttgg aaggtgatgc
      541 cacgcgatcc cccgaggggc acccggatct tctcggtgtt gaagcctttg gtggggacac
      601 tctggacaaa ctccttgaac ttgaggcggt aaaggaggga ggtctttcca gcagagtcca
      661 gcccaatgac cacgacatgc agggcttgga agtggggcaa gaaggaggag gcagtgggcg
      721 ccatctcagt caagtggttc cccatggttc tatctccttc taagcctgct tttttgtaca
      781 aacttgtgat attccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      841 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      901 ac
//
