LOCUS       sequence-118187-        1006 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of hArl6 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL6
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(665..689)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(721..740)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(722..740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(785..985)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(814..834)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctatcat gtcttcacag tctggatctg atcttgaagc
      121 cagtctacac cttcttgcaa gccttctcct tttatggcat cactagcaca aatatgccag
      181 ggtttatctt tgatgttctc taaacacagc aactgagaca cttttacaga tgtcactgca
      241 tctctaagat ccattttatt tgcaaagaat aagattggaa ttcgacggtg tttaatatct
      301 ggatgattca gaagagtatc gagttcttct ttggccacaa ccattcttaa tctatcacta
      361 ctatcaatga caaaaataat agcttggcct tctttataat agtgttccca gagatttctg
      421 tatcttcctt gacctgacat gtcaaacact gtaaatgaca aactggatga tttgaatttc
      481 tctatgctga atcctattgt tggaaggata ttttgagatt gagcatttga aggtttaagt
      541 ttgttaatga tcgtcgtttt gccactatta tctagcccaa ggcacaaaac atgaacctcc
      601 ttcttcttca ggccaagcaa gactgaaagt ctgtctagca atcccatggt tctatctcct
      661 tctaagcctg cttttttgta caaacttgtt tgatcaagct taactagcca gcttgggtct
      721 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      781 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      841 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      901 cattgacgtc aatggggtgg agacttggaa atcccgtgag tcaaaccgct atcccgccca
      961 tggatgtact gccaaaccgc atcacatggt aatagcgatg actata
//
