LOCUS       sequence-118312-       12997 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD
  TITLE     Regulation of mRNA translation during mitosis.
  JOURNAL   Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957.
  PUBMED    26305499
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-08-25"; volume: "4. doi"; pages: " 10.7554/eLife.07957"
COMMENT     Sequence Label: pHR-mCherry-Emi1_ARHGAP5 UTRs
FEATURES             Location/Qualifiers
     source          1..12997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1027..1049
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1087..1105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1173..1277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1278..1346,1347..2138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1496..1515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2309..2897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2798..2817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3051..3068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3138..3158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3143..3472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3323..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4606..4671
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4801..4821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5246..5380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5283..5302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5337..5356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5549..6182
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6229..6354
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6846..7079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7264..7308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7593..7710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7863..8270
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8616..8625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8622..9329
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8767..8785)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9035..9054
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    12407..12995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12460..12480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12878..12889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata
     6721 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     6841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     6901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     6961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     7501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     7561 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     7621 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     7681 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     7741 gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa aagattttat
     7801 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctg
     7861 cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag aagttcagat
     7921 caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg cggtgagcag
     7981 tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc ccggcccgag
     8041 gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta agacccatca
     8101 gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg aattaaccaa
     8161 tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc
     8221 acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg ccaatcaatt
     8281 ggctcggtga gcgcgccgag gaagagaggc gagcggagag tggaggagga ggcggcggcg
     8341 gcgggagcgg tccccaggaa tgtcgctgcc gccgccaccg ccggggccgc tgccgttgag
     8401 gaggagacgg aggagaccga cgttgttagg aagatgatcc ctatgatctt gaagatgttt
     8461 ctgcacagaa atgagggaaa tacaaagaac caaatacagt tctgaaattt gggatctgta
     8521 ttttgagatg attttatttt cagaatgaga agcatatctg gttaccttta tgaatgtaga
     8581 gacatgagaa gagagttcgt acgacgctca cgcgtgccac catggtgagc aagggcgagg
     8641 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     8701 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     8761 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     8821 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     8881 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     8941 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     9001 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     9061 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     9121 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     9181 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     9241 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     9301 ccaccggcgg catggacgag ctgtacaagg gatccggtgg tggtggttct ggtggtggta
     9361 tgagccggcg cccctgcagc tgcgccctac ggccaccccg ctgctcctgc agcgccagcc
     9421 ccagcgcagt gacagccgcc gggcgccctc gaccctcgga tagttgtaaa gaagaaagtt
     9481 ctaccctttc tgtcaaaatg aagtgtgatt ttaattgtaa ccatgttcat tccggactta
     9541 aactggtaaa acctgatgac attggaagac tagtttccta cacccctgca tatttggaag
     9601 gttcctgtaa agactgcatt aaagactatg aaaggctgtc atgtattggg tcaccgattg
     9661 tgagccctag gattgtacaa cttgaaactg aaagcaagcg cttgcataac aaggaaaatc
     9721 aacatgtgca acagacactt aatagtacaa atgaaataga agcactagag accagtagac
     9781 tttatgaaga cagtggctat tcctcatttt ctctacaaag tggcctcagt gaacatgaag
     9841 aaggtagcct cctggaggag aatttcggtg acagtctaca atcctgcctg ctacaaatac
     9901 aaagcccaga ccaatatccc aacaaaaact tgctgccagt tcttcatttt gaaaaagtgg
     9961 tttgttcaac attaaaaaag aatgcaaaac gaaatcctaa agtagatcgg gagatgctga
    10021 aggaaattat agccagagga aattttagac tgcagaatat aattggcaga aaaatgggcc
    10081 tagaatgtgt agatattctc agcgaactct ttcgaagggg actcagacat gtcttagcaa
    10141 ctattttagc acaactcagt gacatggact taatcaatgt gtctaaagtg agcacaactt
    10201 ggaagaagat cctagaagat gataaggggg cattccagtt gtacagtaaa gcaatacaaa
    10261 gagttaccga aaacaacaat aaattttcac ctcatgcttc aaccagagaa tatgttatgt
    10321 tcagaacccc actggcttct gttcagaaat cagcagccca gacttctctc aaaaaagatg
    10381 ctcaaaccaa gttatccaat caaggtgatc agaaaggttc tacttatagt cgacacaatg
    10441 aattctctga ggttgccaag acattgaaaa agaacgaaag cctcaaagcc tgtattcgct
    10501 gtaattcacc tgcaaaatat gattgctatt tacaacgggc aacctgcaaa cgagaaggct
    10561 gtggatttga ttattgtacg aagtgtctct gtaattatca tactactaaa gactgttcag
    10621 atggcaagct cctcaaagcc agttgtaaaa taggtcccct gcctggtaca aagaaaagca
    10681 aaaagaattt acgaagattg tgacggtccg aatgatacta caatttaaga ttatcttatg
    10741 tgtattatag taaatagatg attttcagat tcaaggctcc taagagtttg atttgctctg
    10801 ttttttccta aaataaatat tgtctctccc aactgttaag ttctaggtat tgtacttcca
    10861 attttaactt cagaaccaag atgttggcat gaaccaggct gctgttgaag tacatgtata
    10921 ttataaatta tcttatttgt gttatactct tacatgttat cttttctaag aaaacaaagt
    10981 ccctattatt cctattgcaa agcacacagg aattaagaaa gtacagtaat ttttaaaaaa
    11041 aaatccggta aatgtagtat tcttaacctg ttctatatta cttataccta ttgtctatat
    11101 agctttaatt tatagttgtc agtttaacta ttggcatgtc tggcaaagaa aattaaactt
    11161 taagagtttt ataaactgtt tctaggttgc taaagaattt atttttctac tatatatggt
    11221 atagacaaag catcaaacta tgtacaggaa aaaagcctga ctatttctat ttggaagtag
    11281 gctgaaaaga gaattttcaa aactgttcgt gtcttcagtt cattctgtca taactttgct
    11341 attgtaatat gtgaatacca gtttatttaa gctgttctct tttatactgt attaatttaa
    11401 tgttcatctg cgtttagtac catttttgtt attaaaactg gcatttaccg tttttcacat
    11461 taacccacct tgcaccttcc cccaaactta tctccacttt tctatgcatt ctatcattga
    11521 tttgacacac ttcatagtga gtcatttaaa tactctacgt ttggttcaat taaccagtag
    11581 gttacagtta ttgaaaatta aagtacagtt taaagctcag tctgttacac tgaattgatt
    11641 gtgtttgttt ttgccaaggg tttagatatg cttttaaata ttagaaacat ctaagaacag
    11701 aataacataa ttaaactttt ttctggtaag ttactggaag gtttcactgt ttagggacct
    11761 atcatatgag acttcttaaa ggattaaaag aataggatag tctcataatt gtgagtaaac
    11821 atcaaggcat tatattttac aatactgaat aaaatttcat ctacacacat gttgccattg
    11881 tttcatttaa ggttcagtgc ttatagttaa ctacaatatt ggacctaaca ggatctagat
    11941 tagcaatata aagaagcata gtggtactct gtttcacact ttcagtagat ttattagaag
    12001 tcaaattcta ttcaacagac acttattagg atatacaact aatttaagaa taaaattcca
    12061 ggcacaatat atttttttta aatggtattt gttagtagtg cttcttcccc ttaacattta
    12121 cagtgtaaat actgcaggta accgcaatct aagttagcca aaaagcagct ttttttccca
    12181 tactgtatgt aaataatgta gacctgggtt tttttgttta tttgggtttg tttttttttt
    12241 tgaggtactg gaatctaatt aatatctctt aggtatcaac aaaagggaac aattggaatg
    12301 agaatttagg ccttagcttc catggtgatt tttagttttt tatacaggcg gccgcgactc
    12361 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
    12421 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    12481 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    12541 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    12601 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    12661 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    12721 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    12781 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
    12841 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    12901 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    12961 gacgagtcgg atctcccttt gggccgcctc cccgcat
//
