LOCUS       sequence-118437-         847 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression of hArf6 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARF6-V5-His6
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(668..692)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(721..740)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(722..740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(785..836)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(814..834)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tactcatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa ccgcgggccc tctagatcaa ccactttgta caagaaagct
      121 gggtcagatt tgtagttaga ggttaaccat gtgagcccct catagagtcc gtcccctgag
      181 gtggcacagg agggctgcac ataccagttc ctgtcccgaa tccgggtcag gcccagtttc
      241 tcctggatct cgtggggttt catggcatcg ggcaggtcct gcttgttggc gaagatgagg
      301 attatggcgt ccctcatctc ccggtcattg ataatgcggt gcagctcctg gcgagcctca
      361 tcgatgcggt cgcggtcggc gcagtccact acgaagatga gaccttgggt cccagtgtag
      421 taatgccgcc agagcggccg gatcttgtcc tggccgccca catcccatac gttgaacttg
      481 acatttttgt aagtcaccgt ctccacgttg aaacccacag tgggaatggt ggtcaccgac
      541 tggcccagct tcaacttgta caggattgtt gtcttgccgg ccgcgtccag gcccaacatg
      601 aggatccgca tttccttgtt cccgaagatt ttggatagca ccttccccat ggttctatct
      661 ccttctaagc ctgctttttt gtacaaactt gttgatagct taactagcca gcttgggtct
      721 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      781 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      841 gggcgga
//
