LOCUS       sequence-118455-         895 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of hArl6 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL6-V5-His6
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(696..720)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(749..768)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(750..768)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(813..864)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(842..862)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag atcaaccact ttgtacaaga aagctgggtc
      121 tgtcttcaca gtctggatct gatcttgaag ccagtctaca ccttcttgca agccttctcc
      181 ttttatggca tcactagcac aaatatgcca gggtttatct ttgatgttct ctaaacacag
      241 caactgagac acttttacag atgtcactgc atctctaaga tccattttat ttgcaaagaa
      301 taagattgga attcgacggt gtttaatatc tggatgattc agaagagtat cgagttcttc
      361 tttggccaca accattctta atctatcact actatcaatg acaaaaataa tagcttggcc
      421 ttctttataa tagtgttccc agagatttct gtatcttcct tgacctgaca tgtcaaacac
      481 tgtaaatgac aaactggatg atttgaattt ctctatgctg aatcctattg ttggaaggat
      541 attttgagat tgagcatttg aaggtttaag tttgttaatg atcgtcgttt tgccactatt
      601 atctagccca aggcacaaaa catgaacctc cttcttcttc aggccaagca agactgaaag
      661 tctgtctagc aatcccatgg ttctatctcc ttctaagcct gcttttttgt acaaacttgt
      721 tgatagctta actagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc
      781 cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt
      841 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt ttgga
//
