LOCUS       sequence-118483-         664 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses malE-ELP[V-60] in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-ELP[V-60]
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..265)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(293..320)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(412..498)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(514..531)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
       61 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      241 tgaatgtatt tagaaaaata aacaacggac cgaaagagtt tgtagaaacg caaaaaggcc
      301 atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg ggcgtcctgc
      361 ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct
      421 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
      481 agcctttcgt tttatttgaa gctttcatta ttaatgatgg tggtgatgat gtcccgagcc
      541 ggggactcct acccccggaa cccccactcc gggaacccct actccgggca ctcctactcc
      601 tggaacgcct acccctggaa ctcctacgcc aggtacaccg actcccggga caccaactcc
      661 cggt
//
