LOCUS       sequence-118494-         531 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses malE-AB12 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-AB12
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(145..235)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(263..290)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(382..468)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(483..500)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
       61 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaacggac
      241 cgaaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg
      301 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac
      361 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      421 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgaa gcttcattat
      481 taatgatggt ggtgatgatg tcccgagcct gcgttgcccc cgcccattcc a
//
