LOCUS       sequence-118544-         810 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses malE-GLP-1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-GLP-1
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..280)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(308..335)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(427..513)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(529..546)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa cggaccgaaa gagtttgtag
      301 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
      361 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
      421 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      481 ccagtctttc gactgagcct ttcgttttat ttgaagcttt cattattaat gatggtggtg
      541 atgatgtccc gagccacgcc ctttaacgag ccaggcgata aattcttttg cagcctgctc
      601 ttctaagtaa ctagatacat cagaagtgaa tgtcccttca ccatgggcac ccctaccttt
      661 cacaagccaa gcaataaact ccttcgcagc ttgctcttcg agataggatg atacgtcact
      721 cgtaaaagta ccttctccgt gcgctccgcg acctttgact agccacgcta taaactcttt
      781 agccgcttgt tcctctaaat aagatgacac
//
