LOCUS       sequence-118705-         833 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-11. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: YFP(159-238)-gamma-11 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(283..304)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(526..545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(527..545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(590..793)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(619..639)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtgatagat ctttatgaaa taacacagct gcctttttct ttaaaggggt tcttgtcttc
       61 tggaattccc tttactagag ggtcctctcc agaacgttct tcaatatagt tctttatttc
      121 ttcagaacat ttagacactt gttgtctctg caacttcact tctttgcgaa gctgctcaac
      181 ttccattttc agtttttcct tctctggcaa atcttcgatg tgaagggcag gcatggatct
      241 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      301 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg
      361 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      421 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      481 cttgcccatg gtggcggatc cgagctcggt accaagcttg ggtctcccta tagtgagtcg
      541 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
      601 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      661 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      721 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      781 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caa
//
