LOCUS       sequence-118774-         800 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of shRNA targeting human ARF1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Volpicelli-Daley LA, Li Y, Zhang CJ, Kahn RA
  TITLE     Isoform-selective effects of the depletion of ADP-ribosylation 
            factors 1-5 on membrane traffic.
  JOURNAL   Mol Biol Cell. 2005 Oct;16(10):4495-508. Epub 2005 Jul 19.
  PUBMED    16030262
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-10"; volume: "16(10)"; pages: "4495-508. Epub 
            2005 Jul 19"
COMMENT     Sequence Label: pSUPER-ARF1a
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     92..108
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     93..109
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(182..396)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(242..261)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(416..432)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(416..432)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(464..483)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(465..483)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(490..507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(490..506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(499..521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(735..754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta
       61 aagggaacaa aagctggtac cgggcccccc ctcgaggtcg acggtatcga taagcttttc
      121 caaaaaaccg tggagtacaa gaacatctct tgaatgttct tgtactccac ggtggggatc
      181 tgtggtctca tacagaactt ataagattcc caaatccaaa gacatttcac gtttatggtg
      241 atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc tgtccctcac
      301 agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc
      361 gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat tcctgcagcc cgggggatcc
      421 actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta
      481 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      541 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      601 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      781 tccccgtcaa gctctaatcg
//
