LOCUS       sequence-118798-         849 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..801)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
ORIGIN
        1 ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgaattc
       61 acgcgtttag cgagatccgg tggagccggg tccggcggtg ccgtccacgg cagaattgga
      121 cgactgagcg cgggatctgg cgaaggcgat gggggtcttg aaggcgtgct ggtactccac
      181 gatgcccagc tcggtgttgc tgtgcagctc ctccacgcgg cggaaggcga acatggggcc
      241 cccgttctgc aggatgctgg ggtggatggc gctcttgaag tgcatgtggc tgtccaccac
      301 gaagctgtag tagccgccgt cgcgcaggct gaaggtgcgg gcgaagctgc ccaccagcac
      361 gttatcgccc atggggtgca ggtgctccac ggtggcgttg ctgcggatga tcttgtcggt
      421 gaagatcacg ctgtcctcgg ggaagccggt gcccaccacc ttgaagtcgc cgatcacgcg
      481 gccggcctcg tagcggtagc tgaagctcac gtgcagcacg ccgccgtcct cgtacttctc
      541 gatgcgggtg ttggtgtagc cgccgttgtt gatggcgtgc aggaaggggt tctcgtagcc
      601 gctggggtag gtgccgaagt ggtagaagcc gtagcccatc acgtggctca gcaggtaggg
      661 gctgaaggtc agggcgcctt tggtgctctt catcttgttg gtcatgcggc cctgcttggg
      721 ggtgccctct ccgccgccca ccagctcgaa ctccacgccg ttcagggtgc cggtgatgcg
      781 gcactcgatc tccatggcgg gcaggccgct ctcgtcgctc tccatggttg tacacaattc
      841 tttgccaaa
//
