LOCUS       sequence-118982-         987 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of hArf4 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF4-GFP
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(641..665)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(761..964)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(790..810)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcacgtttt gaaagctcat ttgacagcca gtcaagtcct
      121 tcatacagac cagttccttg tgttgcacaa gtggcttgaa cataccatgt tctgttacga
      181 agagactgaa gccctagttt atctgtcatt tcactgatgg caatagcatt tggcaaatcc
      241 tgtttgtttg caaaaggtag cagcactgca tctctcaatt catctaccag aagcattttc
      301 tgcagctcat ctgctacttc ctgaattctt tcacgatcgt tgctatctac cacaaaaata
      361 agaccctggg tattctggaa gtaatgcttc cagagaggcc taattctatc ttgaccacca
      421 acatcccata ctgtgaaaca aatgttctta tattctactg tttccgcatt aaaaccaatg
      481 gtaggaatgg tggtgactat ctcccctaac ttcagtttat acagaatggt tgtcttgcca
      541 gcagcatcca atccaaccat caaaatgcgc atctgcttct tgccaaatag tcgggagaag
      601 agggaggaga tagtgaggcc catggttcta tctccttcta agcctgcttt tttgtacaaa
      661 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      721 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      781 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      841 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      901 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      961 tcaccatggt aatagcgatg actaata
//
