LOCUS       sequence-119162-         868 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses RNA-binding protein MS2 with fast SNAP fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Carrocci TJ, Hoskins AA
  TITLE     Imaging of RNAs in live cells with spectrally diverse small molecule
            fluorophores.
  JOURNAL   Analyst. 2014 Jan 7;139(1):44-7. doi: 10.1039/c3an01550e. Epub 2013 
            Nov 1.
  PUBMED    24187681
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3an01550e"; journalName: "Analyst"; date: "2014-01-7- 7"; 
            volume: "139"; issue: "1"; pages: "44-7"
COMMENT     Sequence Label: MS2-Snapf
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             134..145
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac accaagaact
       61 tagtttcgac ggatccacca tgccaaaaaa gaaaagaaaa gttggctacc cctacgacgt
      121 gcccgactac gccatcgaag gccgccatat gctagccgtt aaaatggctt ctaactttac
      181 tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt
      241 cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac
      301 ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc
      361 taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa
      421 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
      481 tccctcagca atcgcagcaa actccggcat ctacgcggcc gcaggaggat caggaggatc
      541 aatggacaaa gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga
      601 actgtctggg tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc
      661 cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat
      721 gcaggccacc gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc
      781 tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg
      841 gaaactgctg aaagtggtga agttcgga
//
