LOCUS       sequence-119238-         700 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  An amino-terminal CFP Fragment was fused to residues 202-477 of 
            RGS7. When co-expressed with a carboxyl terminal fragment of CFP 
            fused to Gbeta-5, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: CFP(1-158)-RGS7t
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(85..87,88..90,91..549)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(130..151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(391..410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
       61 ggtaccgagc tcggatccgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      121 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      181 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      241 aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc
      301 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      361 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      421 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      481 gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat
      541 atcaccgccg acaagcagag atccatcgca acccccgtgc ctggatgtgt aaatacaact
      601 gaagtggaca ttaagaagtc atccagaatg agaaaccccc acaaaacacg gaagtctgtc
      661 tatggtttac gaaatgatat tagaagtcac agtcctaccc
//
