LOCUS       sequence-119447-         754 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Induce human iN conversion from fibroblast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Lau S, Rylander Ottosson D, Jakobsson J, Parmar M
  TITLE     Direct neural conversion from human fibroblasts using 
            self-regulating and nonintegrating viral vectors.
  JOURNAL   Cell Rep. 2014 Dec 11;9(5):1673-80. doi: 
            10.1016/j.celrep.2014.11.017. Epub 2014 Dec 4.
  PUBMED    25482564
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.11.017"; journalName: "Cell Rep"; date: 
            "2014-12-11- 11"; volume: "9"; issue: "5"; pages: "1673-80"
COMMENT     Sequence Label: 1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4
            Xma_lo...
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..56)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             291..524
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    596..717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(633..652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     687..706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
       61 cggaccttcc ttcccgcggc ctgctgccgg ctctagataa tggcattcac cgcgtgcctt
      121 aattcgaatg gcattcaccg cgtgccttaa acgcgttggc attcaccgcg tgccttaaat
      181 gcattggcat tcaccgcgtg ccttaacccg gtcgagctcg gtacctttaa gaccaatgac
      241 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
      301 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
      361 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      421 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      481 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
      541 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
      601 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      661 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      721 tgtctggctc tagctatccc gcccctaact ccgc
//
