LOCUS       sequence-119570-        1021 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of hArl5 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL5A-V5-His6
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(569..593)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             619..660
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             670..687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(710..727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    716..941
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atgggaattc tcttcactag aatatggaga
       61 ctgttcaatc accaggagca caaagttatc attgttgggt tggataatgc agggaaaact
      121 accattcttt accaattttc tatgaatgaa gttgtacata catctcctac aataggaagt
      181 aatgtagaag agatagtgat taataataca cgtttcctaa tgtgggatat tggtggccaa
      241 gaatctcttc gttcttcctg gaacacttac tatactaaca cagagtttgt aatagttgtt
      301 gtggacagta cagacagaga gaggatttct gtaactagag aagaactcta taaaatgtta
      361 gcgcatgagg acctaagaaa agctggattg ctgatttttg ctaataaaca agatgttaaa
      421 gaatgcatga ctgtagcaga aatctcccag tttttgaagc taacttctat taaagatcac
      481 cagtggcata tccaggcatg ctgtgctcta actggcgagg gattgtgcca aggacttgaa
      541 tggatgatgt cacgacttaa gattagagac ccagctttct tgtacaaagt ggttgatcta
      601 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      661 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
      721 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      781 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      841 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggga
      901 agacaatagc aggcatgctg gggatgcggt gggctctatg ggcttctgag gcggaaagaa
      961 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcccatta agcgcggcgg
     1021 g
//
