LOCUS       sequence-119575-         910 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of hArl14 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL14-V5-His6
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(607..631)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             657..698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             708..725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(748..765)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    754..865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggttcgct gggttctaaa aatccgcaaa
       61 ccaaacaagc ccaagttctt cttttgggac ttgactcagc tgggaagtct actctccttt
      121 ataaattaaa gcttgctaag gatattacca ccatccctac aataggtttc aatgtggaaa
      181 tgatcgagtt ggaaaggaat ttttcactca cagtctggga tgttggagga caggaaaaaa
      241 tgagaactgt ttggggctgt tactgtgaga acaccgatgg gctggtgtat gttgtggaca
      301 gtacagacaa acagcgactg gaagagtctc agagacagtt tgagcacatt ttgaagaatg
      361 aacacattaa aaatgtgcct gttgttctat tagccaacaa acaagacatg cctggagctc
      421 tgactgctga ggacatcacc agaatgttca aagtgaagaa gctttgcagt gaccggaact
      481 ggtatgtgca accctgctgt gccctcacag gggaggggct ggcccagggg ttcaggaaat
      541 taactggatt tgtgaagagc cacatgaaat caagaggaga cactttggcg ttcttcaagc
      601 agaacgaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta
      661 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
      721 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      781 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      841 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
      901 tggggtgggg
//
