LOCUS       sequence-119592-        1045 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV2-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..43)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(204..223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(205..223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(268..367)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(297..317)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     606..625
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        700..804
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1023..1042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccttgctcac gaattcagcg taatctggaa catcgtatgg gtaggatccc tggactggct
       61 tcccataccg aaaatttcga accatggaag tgtgagcctt tctaagagct gccttgttga
      121 gcaggattct caaattagac agcatggtac ctccggatcg ataattctag agggcccgtt
      181 taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      241 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      301 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      361 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      421 tgagtcaaac cgctaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
      481 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
      541 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
      601 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc
      661 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      961 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1021 ctgctatgtg gcgcggtatt atccc
//
