LOCUS       sequence-119597-         798 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV6-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..43)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(205..224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(206..224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(269..320)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(298..318)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     494..513
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        588..692
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttgctcac gaattcagcg taatctggaa catcgtatgg gtaggatccc tggtactggc
       61 ttcccatacc gaaaatttcg aaccatggaa gtgtgagcct ttctaagagc tgccttgttg
      121 agcaggattc tcaaattaga cagcatggta cctccggatc gataattcta gagggcccgt
      181 ttaaacgcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
      241 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
      301 gcctaccgcc catttgcgtc aatacgcgta tatctggccc gtacatcgcg aagcagcgca
      361 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
      421 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
      481 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
      541 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      781 aagtaaaaag atgctgaa
//
