LOCUS       sequence-119839-         818 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  photoactivatable fluorescent calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: spa-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..105
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(109..117,118..132)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             160..216
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     429..450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(556..577)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgcca ccatgggttc tcatcatcat
       61 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      121 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
      181 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaccgcc
      241 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacgt ggaggacggc
      301 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      361 ctgcccgaca accactacct gagccatcag tccgtgcttt cgaaagaccc caacgagaag
      421 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      481 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
      541 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      601 aggggcgagg gtgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
      661 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag
      721 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      781 gaaggctacg tccaggagcg caccatctcc ttcaagga
//
