LOCUS       sequence-119969-         944 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expresses human FbxO5 in mammalian cells with FLAG-HA tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: FLAG-HA-FbxO5-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agccaccatg gactacaaag acgatgacga taaagggggc ggtggaggtt
      121 acccatacga tgttccagat tacgctggtg gaggtggagg ttctagaatg agccggcgcc
      181 cctgcagctg cgccctacgg ccaccccgct gctcctgcag cgccagcccc agcgcagtga
      241 cagccgccgg gcgccctcga ccctcggata gttgtaaaga agaaagttct accctttctg
      301 tcaaaatgaa gtgtgatttt aattgtaacc atgttcattc cggacttaaa ctggtaaaac
      361 ctgatgacat tggaagacta gtttcctaca cccctgcata tttggaaggt tcctgtaaag
      421 actgcattaa agactatgaa aggctgtcat gtattgggtc accgattgtg agccctagga
      481 ttgtagaact tgaaactgaa agcaagcgct tgcataacaa ggaaaatcaa catgtgcaac
      541 agacacttaa tagtacaaat gaaatagaag cactagagac cagtagactt tatgaagaca
      601 gtggctattc ctcattttct ctacaaagtg gcctcagtga acatgaagaa ggtagcctcc
      661 tggaggagaa tttcggtgac agtctacaat cctgcctgct acaaatacaa agcccagacc
      721 aatatcccaa caaaaacttg ctgccagttc ttcattttga aaaagtggtt tgttcaacat
      781 taaaaaagaa tgcaaaacga aatcctaaag tagatcggga gatgctgaag gaaattatag
      841 ccagaggaaa ttttagactg cagaatataa ttggcagaaa aatgggccta gaatgtgtag
      901 atattctcag cgaactcttt cgaaggggac tcagacatgt ctta
//
