LOCUS       sequence-120487-         830 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression construct of human codon-adapted anion 
            channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via 
            a NotI site in the pFUGW vector backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL
  TITLE     Natural light-gated anion channels: A family of microbial rhodopsins
            for advanced optogenetics.
  JOURNAL   Science. 2015 Jun 25. pii: aaa7484.
  PUBMED    26113638
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Jun 25. pii: aaa7484."
COMMENT     Sequence Label: pFUGW-hGtACR1-EYFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(63..776,777..779))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(108..129)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     457..476
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     716..737
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      121 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg
      181 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      241 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      301 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      361 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      421 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      481 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      541 tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc cgtagccgaa ggtggtcacg
      601 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
      661 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      721 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacg
      781 gcggccgcgg tctcgccggc tctggcgtcc tcgtacctct cggtgttgcc
//
