LOCUS       sequence-120517-        1119 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Complementation in Cryptococcus neoformans. Targets DNA constructs 
            to a Safe Haven Site, a small gene-free region. Contains NEO 
            resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA
  TITLE     A genomic safe haven for mutant complementation in Cryptococcus 
            neoformans.
  JOURNAL   PLoS One. 2015 Apr 9;10(4):e0122916. doi: 
            10.1371/journal.pone.0122916. eCollection 2015.
  PUBMED    25856300
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-9"; volume: "10(4):e0122916. doi"; pages: " 
            10.1371/journal.pone.0122916. eCollection 2015"
COMMENT     Sequence Label: pSDMA57
FEATURES             Location/Qualifiers
     source          1..1119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     45..65
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        47..65
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    78..185
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     102..118
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     102..118
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(152..168)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(222..239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(222..238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(231..253)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
       61 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccc
      121 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      181 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      361 agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      421 tttgttaaat cagctcattt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
      481 tcaaaagaat agaccgagat agggttgagt ccatggctgc gaggatgtga gctggagagc
      541 ggcgcacggg aaggggagga ctcacataag catgcaggat tcgagtggca tggtgtgcac
      601 tgagtgtatg gttgtcggag gagaggatga tggtaacaac aatagcagca acgtcactcg
      661 acgcgcgtcc ggtgtgccac acggggtaac gccgagtcgc cgtcagggtc gccgagacca
      721 ctctcacagc gtcaccgttg gcaccagctc agcttacagc ttctatcctc cgccagcatc
      781 cacatacatc ccctataccg catcccccac ccactgccca aggtgagtca tcttcccgcc
      841 cccttccctt gcccgccact cagtcctcca tcctccacta atccacctta tcgcacccac
      901 cgcctatcgc acatccgagc acaatgctgg gcctgccagg ggctgctaga tggtgctctc
      961 cccacgctga tctgcatgcc ggccattgga tcatgggtgc taggtgctgg gtgctggatg
     1021 ttggatgctg gatgctgggt gcacgcttgg tcatttcctt ccaggattga cggtcgccga
     1081 gaggacgacg tggcgttcga caacgagggc cgatagcac
//
