LOCUS       sequence-120519-        1082 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Complementation in Cryptococcus neoformans. Targets DNA constructs 
            to a Safe Haven Site, a small gene-free region. Contains 
            nourseothricin resistance marker (NAT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Arras SD, Chitty JL, Blake KL, Schulz BL, Fraser JA
  TITLE     A genomic safe haven for mutant complementation in Cryptococcus 
            neoformans.
  JOURNAL   PLoS One. 2015 Apr 9;10(4):e0122916. doi: 
            10.1371/journal.pone.0122916. eCollection 2015.
  PUBMED    25856300
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-9"; volume: "10(4):e0122916. doi"; pages: " 
            10.1371/journal.pone.0122916. eCollection 2015"
COMMENT     Sequence Label: pSDMA25
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..30
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14..30
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     49..69
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        51..69
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    82..189
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     106..122
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     106..122
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(156..172)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(197..216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(198..216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(226..243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(226..242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
       61 cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga
      121 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
      181 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
      241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      361 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
      421 aatttttgtt aaatcagctc atttttttaa ccaataggcc gaaatcggca aaatccctta
      481 taaatcaaaa gaatagaccg agatagggtt gagtccatgg ctgcgaggat gtgagctgga
      541 gagcggcgca cgggaagggg aggactcaca taagcatgca ggattcgagt ggcatggtgt
      601 gcactgagtg tatggttgtc ggaggagagg atgatggtaa caacaatagc agcaacgtca
      661 ctcgacgcgc gtccggtgtg ccacacgggg taacgccgag tcgccgtcag ggtcgccgag
      721 accactctca cagcgtcacc gttggcacca gctcagctta cagcttctat cctccgccag
      781 catccacata catcccctat accgcatccc ccacccactg cccaaggtga gtcatcttcc
      841 cgcccccttc ccttgcccgc cactcagtcc tccatcctcc actaatccac cttatcgcac
      901 ccaccgccta tcgcacatcc gagcacaatg ctgggcctgc caggggctgc tagatggtgc
      961 tctccccacg ctgatctgca tgccggccat tggatcatgg gtgctaggtg ctgggtgctg
     1021 gatgttggat gctggatgct gggtgcacgc ttggtcattt ccttccagga ttgacggtcg
     1081 cc
//
