LOCUS       sequence-120552-         458 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a non-targeting gRNA and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, 
            Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, 
            Kim GE, Carpizo DR, Kim SK, Winslow MM
  TITLE     Pancreatic cancer modeling using retrograde viral vector delivery 
            and in vivo CRISPR/Cas9-mediated somatic genome editing.
  JOURNAL   Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. 
            Epub 2015 Jul 15.
  PUBMED    26178787
REFERENCE   2  (bases 1 to 458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.264861.115"; journalName: "Genes Dev"; date: 
            "2015-07-15- 15"; volume: "29"; issue: "14"; pages: "1576-85"
COMMENT     Sequence Label: Lenti-sgNT/Cre
FEATURES             Location/Qualifiers
     source          1..458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..282
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     34..54
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     205..224
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        303..378
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccgctctagc cctgcaggaa ctgtttaaac tgtgagggcc tatttcccat gattccttca
       61 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
      121 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      181 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      241 gatttcttgg ctttatatat cttgtggaaa ggaccaaaca ccgcgaggta ttcggctccg
      301 cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      361 gtggcaccga gtcggtgctt ttttgtttgg cgcgcctatg gcttaattaa catggcggcc
      421 gcattctacc gggtagggga ggcgcttttc ccaaggca
//
