LOCUS       sequence-120628-        7818 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses full-length P. vivax P12p ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: P12p-bio
FEATURES             Location/Qualifiers
     source          1..7818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3198..3253
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3252..3271)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5489..5507)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5575..5679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5680..5748,5749..6540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5898..5917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6711..7299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7200..7219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7453..7470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7587..7608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7623..7628,7629..7646,7647..7653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7661..7677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7666..7688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7685..7701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7685..7701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcagtgc agaaacagca gcagtctagc atccccgcca
     1381 gaaagcaccc cgaggaagaa ggcggaggac ccagccctca gaatgccctg gctcaggtgg
     1441 ccaaccagat gaagggcacc tgtgacttca gcagagcccc cctgaatgtg gcctgcagcg
     1501 agaacgagat cgtggccctg cctggcggag aaggcgctat ggtgtctggc accacaggca
     1561 gcaccgccaa cgatgagagg gccagacact gcgtgcagtt caccaagggc ttcgacgtgc
     1621 tgaccttcgt gtgccccaag cggagcagcg aggattacag cggcgtggaa atccggccca
     1681 tgagctgctt cgagacagtg cggagaaccg acggcaccaa ccagcagctg agcgaggtgc
     1741 tgaaaggcgt gcagctggaa aaccgggaca ccgacctgct gagcatccgg cgggtgttca
     1801 tcccccccac catctaccag aacttcatct tcgagtgcag ctgcgacaac agcctgacct
     1861 tctggaagaa caagatgggc gccagaggca tcatgcgggt gcacctgaga agaaacatcc
     1921 tgttcggctg cgacttcgac cacaccggcg gagtggagta tacaggcgga ctgggcggag
     1981 agctgcccat ggctgatgaa gcctctgtgg gcagcgccta cgacagatct gctggcggag
     2041 gaagcgccga ggatagaagt gctgggggcg gatctgccga cgactgggcc ttttggagaa
     2101 acgccggacc ctccgccgag gaactggccg agaaaaacaa ggccgccttc accaacttct
     2161 acccccctgg cgaagtgtcc ctggccaaag aaaagggcct cgtgtgcgac gtgaagatca
     2221 ccaagagaga ggtgtacctg ggcctcgtgt gtccccctgg ctacgagatg taccccagca
     2281 actgctttga gagagtgctg cagaaagacg gctccatcgt gcgggtgtcc gagctgctga
     2341 agcacgacgt gtcctttcac gccgacggca accggcggat gagcttcgcc accttcaccc
     2401 tgaaccggaa cgagaacccc cagggcttca gctgcatgtg cctgaacgtg caggcccctg
     2461 aagccccacc cctgcaggct aatttcgcct tccacaacta cgagtctgcc ggcgtcagat
     2521 tcggactgcc aggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     2581 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     2641 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     2701 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     2761 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     2821 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     2881 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     2941 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     3001 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     3061 ccaagatcca ggttttatcc aaagggttga attccggatc actgcatcat attctggatg
     3121 cacagaaaat ggtgtggaat catcgttaag tctagacacg tgattaatta aggatccccc
     3181 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3241 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3301 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3361 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3421 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3481 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3541 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3601 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3661 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3721 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3781 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3841 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3901 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3961 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     4021 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     4081 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     4141 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     4201 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     4261 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     4321 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4381 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4441 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4501 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4561 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4621 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4681 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4741 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4801 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4861 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4921 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4981 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5041 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5101 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     5161 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     5221 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     5281 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     5341 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5401 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5461 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5521 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5881 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7501 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7561 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7621 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7681 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7741 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7801 agaactggta ggtatgga
//
