LOCUS       sequence-120650-        7509 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. 
            C-terminal rat Cd4d3+4 tag, biotinylation sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, 
            Rayner JC
  TITLE     A Library of Plasmodium vivax Recombinant Merozoite Proteins Reveals
            New Vaccine Candidates and Protein-Protein Interactions.
  JOURNAL   PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0004264. doi: 
            10.1371/journal.pntd.0004264. eCollection 2015 Dec.
  PUBMED    26701602
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2015-12-23"; volume: "9(12):e0004264. doi"; 
            pages: " 10.1371/journal.pntd.0004264. eCollection 2015 Dec"
COMMENT     Sequence Label: MTRAP-bio
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2889..2944
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2943..2962)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5180..5198)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5266..5370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5371..5439,5440..6231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5589..5608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6402..6990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6891..6910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7144..7161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7278..7299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7314..7319,7320..7337,7338..7344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7352..7368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7357..7379
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7376..7392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7376..7392
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaga gaatcggcaa gagaaagtgc gagcagtggg
     1381 actcttggag cgcctgcaag gacggcatca gcaccagagt gtgcctgacc aacaaggccg
     1441 tgaccgacaa gatgacctgc aaggcctgca acatctgggg cgattggagt gcctgtaaaa
     1501 acggcaagcg gcaccggaag gtcgtgaact gccccttcat cagagaggaa caggactgcg
     1561 accctaacaa ggccaacaag cagaacgccc ggaacaacgc cgccatctac ttcaacaacg
     1621 acgactacga cgacgagcag gacgacgtgt tcgaggaaac cctgcaggaa gagtccaacc
     1681 tgcccgtgaa gggcgacagc agcgagccca tcttcctgga acaggatagc cacggcgagg
     1741 gcaagcagca gagcatgagc gagagcttcg cccacgtgga cgaggtggac gctacagccc
     1801 agccccacaa tgaggtgctg accgacacca ccacacccag cgaagcctct ccccctgccg
     1861 acgacgccaa caatgaggcc tacgtgtcct accccgagga agaacctctg cacggccccc
     1921 agaacaatgc cgatgaagct caggctggcg ccgaccacct gagcagccat aacctggccg
     1981 acgaccaggc cgactctcag gccgatggcc agacaaatgc cgcccctagc tctgagccca
     2041 gagccaagca cttccgggac cacaagttcg aggacgtgtc cagccctcct ggcgagggcc
     2101 agaatgagaa tcacgccgag ggaaacacca accagaacag cttctacgag cagcagggca
     2161 gccaccacga gcacagcgaa cacaagtggc ggaagaagca caacgctggc gctggatctg
     2221 gcggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct
     2281 ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga
     2341 aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc
     2401 agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga
     2461 taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag
     2521 ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca
     2581 ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg
     2641 agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag
     2701 caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc
     2761 aggttttatc caaagggttg aattccggat cactgcatca tattctggat gcacagaaaa
     2821 tggtgtggaa tcatcgttaa gtctagacac gtgattaatt aaggatcccc cgacctcgac
     2881 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2941 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     3001 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     3061 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     3121 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     3181 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     3241 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     3301 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     3361 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     3421 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     3481 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     3541 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     3601 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     3661 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     3721 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3781 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3841 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     3901 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     3961 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     4021 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     4081 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     4141 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     4201 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     4261 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     4321 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     4381 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     4441 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     4501 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     4561 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4621 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4681 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4741 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4801 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     4861 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     4921 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4981 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5041 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5101 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     5161 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5221 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5581 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5881 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     5941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7381 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     7441 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     7501 aggtatgga
//
