LOCUS       sequence-120693-         796 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag 
            and driven by SV40 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Chen X, Bieker JJ
  TITLE     Unanticipated repression function linked to erythroid Kruppel-like 
            factor.
  JOURNAL   Mol Cell Biol. 2001 May;21(9):3118-25.
  PUBMED    11287616
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2001-05"; volume: "21(9)"; pages: "3118-25"
COMMENT     Sequence Label: pSG5-FLAG-mEKLF Zinc Fingers
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(394..417)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(428..444)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(428..444)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(450..469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(451..469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(497..516)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 agttctgctt taataagatc tggatccgaa ttagcttgat atcgaattcc tgcagcccga
       61 tccactctta tttcatcccc agtccttgtg caggatcact cagaggtgac gcttcatgtg
      121 cagagctaag tggtcagagc gtgaaaaagc acgtgggcag aggccacagc agaagggacg
      181 atgtccagtg tgcttccggt agtggcgcgt cagttcgtct gagcgagcga acctccagtc
      241 acagccgtcc caggagcagg cataaggctt ctctcccgtg tgcgtgcgca ggtgcgcctt
      301 gaggtgcgag ctcttggagt agctcttccc gcagctttcg tgcccgcacg tatgtgccgc
      361 ctgcctcttg ctagctgagg ccatgggggg atctttgtca tcgtcgtcct tgtagtccat
      421 ggtggcggat ccactagttc tagaattcgc cctatagtga gtcgtattac aattctttgc
      481 caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg aaaaagaaga
      541 aggcatgaac atggttagca gaggggcccg gtttggactc agagtatttt atcctcatct
      601 caaacagtgt atatcattgt aaccataaag agaaaggcag gatgatgacc aggatgtagt
      661 tgtttctacc aataagaata tttccacgcc agccagaatt tatatgcaga aatattctac
      721 cttatcattt aattataaca attgttctct aaaactgtgc tgaagtacaa tataatatac
      781 cctgattgcc ttgaaa
//
