LOCUS       sequence-120754-        1056 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of SDR5C1 (HSD17B10), full coding sequence + 
            N-term. His tag (thrombin site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W
  TITLE     RNase P without RNA: identification and functional reconstitution of
            the human mitochondrial tRNA processing enzyme.
  JOURNAL   Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013.
  PUBMED    18984158
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.09"; journalName: "Cell"; date: "2008-10-31- 
            31"; volume: "135"; issue: "3"; pages: "462-74"
COMMENT     Sequence Label: pET28-HADH2
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(844..861)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(871..888)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             907..929
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    944..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(968..987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(969..987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtcaagg
       61 ctgcatacga atggccccat ccagccggat gacctctcca ttgaggaatg ggttctcgat
      121 gatggcctgt acgaggtgag catactcagc agggtcaccc agtcggctag ggaagggcac
      181 ttggctggcc aagaagttgc acactttctc tgggaggctg gtcagcagtg gggtgccaaa
      241 cagacctggg gcaatggtca tcacccggat acctatggga gccagatccc gagcaatggg
      301 cagtgtcatg cccactattc cccccttgga agcagagtat gcagcttgtc caacctgacc
      361 ctcgaaggca gccacactgg cagtgttgat gatgacccca cgttggcctc cctggtctgg
      421 ttcattctgg cccatctcac cagccaccag gcggatcaca ttgaaggtgc ccatgagatt
      481 cacatcaaga actcgctgga agtcttccaa ggtatgggtc tggcccttct ttaagttgta
      541 cgtcttgcta gccaccgcga tgcctgcaca gttgacagct acatccacac ggccaaactt
      601 tccttttgct agagccagag ctgtttgcac atccttctca gaggtcacgt cggctggggc
      661 gaaaacgcag ttgtttccta acttcttggc ttgggcctcc ccacccgagt tgggcaggtc
      721 cagaagcaca gcagaggctc cctgccccac aagtcgctcc gccgtggcca ggcccaggcc
      781 cgaggctcct ccggttatta ccgccaccag gcccttcacg ctccgacacg ctgctgccat
      841 atggctgccg cgcggcacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat
      901 ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      961 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
     1021 cggacgcatc gtggccggca tcaccggcgc cacagg
//
