LOCUS       sequence-120776-        3249 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Pasteurella multocida Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3249)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX406 Pasteurella multocida Cas9
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             2289..2297
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accggtgcca ccatgtaccc atacgatgtt ccagattacg cttcgccgaa gaaaaagcgc
       61 aaggtcgaag cgtcccagac taccaacctg agttacatcc tgggactgga cctgggaatc
      121 gcaagtgtcg gctgggctgt cgtggaaatc aatgaaaacg aggaccctat cggcctgatt
      181 gatgtggggg tccgaatctt cgaacgagca gaggtgccaa agaccggaga gtccctggca
      241 ctgtctcgga gactggccag atccacaagg cgcctgattc gacggagagc tcacaggctg
      301 ctgctggcaa agagatttct gaaaagggag ggaatcctgt ctaccattga cctggaaaag
      361 ggcctgccca accaggcctg ggaactgaga gtggctggcc tggagaggcg cctgagtgct
      421 attgaatggg gggcagtcct gctgcacctg atcaaacatc gcggatacct gagcaagcga
      481 aaaaacgagt cccagaccaa caataaggaa ctgggagcac tgctgtccgg agtggcacag
      541 aatcaccagc tgctgcagtc tgacgattac agaacacctg ccgagctggc tctgaagaaa
      601 ttcgctaagg aggaaggcca catccgcaac cagcgagggg catataccca taccttcaac
      661 aggctggacc tgctggccga gctgaatctg ctgttcgctc agcagcatca gtttggcaat
      721 ccacactgca aagagcatat ccagcagtac atgaccgaac tgctgatgtg gcagaagccc
      781 gccctgtcag gagaggctat tctgaagatg ctgggcaaat gtacacacga gaagaacgag
      841 ttcaaggccg ctaaacatac ttatagcgcc gagcgatttg tgtggctgac caagctgaac
      901 aatctgagaa tcctggagga tggagcagag agggccctga acgaggaaga gcgccagctg
      961 ctgattaatc acccctacga gaagagtaaa ctgacctatg cccaggtgcg caagctgctg
     1021 ggcctgtcag aacaggctat cttcaagcac ctgcggtact ctaaagaaaa cgctgagagt
     1081 gcaaccttca tggagctgaa agcctggcat gctattagaa aggccctgga aaatcaggga
     1141 ctgaaggaca cttggcagga tctggcaaag aaaccagacc tgctggatga gattggcaca
     1201 gccttctccc tgtacaaaac tgacgaagat atccagcagt atctgacaaa taaggtgccc
     1261 aactccgtca ttaatgccct gctggtgtct ctgaacttcg acaagttcat cgagctgtct
     1321 ctgaagagtc tgaggaaaat cctgccactg atggaacagg ggaagcggta cgatcaggct
     1381 tgcagagaaa tctacggcca ccattatggg gaagcaaatc agaaaactag ccagctgctg
     1441 ccagcaatcc cagcacagga gattaggaac ccagtggtcc tgcgaaccct gagccaggct
     1501 cgaaaagtga tcaacgcaat cattcgacag tatggctccc ctgctcgggt ccacattgaa
     1561 actggaagag agctgggcaa gtctttcaaa gaacgacggg agatccagaa acagcaggag
     1621 gacaaccgga ccaagagaga aagtgccgtg cagaagttca aagagctgtt cagtgacttt
     1681 agctccgaac ccaagtcaaa agatatcctg aagtttcgcc tgtacgagca gcagcacggg
     1741 aagtgcctgt attctggaaa agaaatcaac attcacagac tgaatgaaaa gggctacgtg
     1801 gagattgatc atgcactgcc tttctcaagg acatgggacg atagctttaa caacaaggtg
     1861 ctggtcctgg ccagcgagaa tcagaacaag gggaatcaga ctccctacga gtggctgcag
     1921 ggaaaaatca actccgaacg gtggaagaat ttcgtggccc tggtcctggg ctcacagtgt
     1981 agcgcagcca agaaacagag actgctgact caggtcatcg acgataacaa gttcattgac
     2041 aggaatctga acgatacccg ctacatcgcc cgatttctga gcaactatat tcaggagaat
     2101 ctgctgctgg tgggcaagaa taagaaaaac gtctttactc ctaacgggca gattaccgca
     2161 ctgctgagat ccaggtgggg actgatcaaa gccagagaga acaataacag gcaccatgct
     2221 ctggacgcaa tcgtggtcgc ttgtgcaacc ccctctatgc agcagaagat cacaaggttc
     2281 attcgcttta aggaggtgca cccttacaaa attgaaaacc gctatgagat ggtcgatcaa
     2341 gaaagtggcg agatcatttc acctcatttc cccgagcctt gggcctactt tcggcaggaa
     2401 gtgaacatca gagtcttcga caatcaccca gataccgtgc tgaaggagat gctgcctgac
     2461 cggccacagg ccaatcatca gttcgtgcag cctctgtttg tcagccgggc tccaacaaga
     2521 aagatgtccg gacagggcca catggagact atcaagtctg caaaaaggct ggccgaaggc
     2581 atcagtgtgc tgcgcattcc actgactcag ctgaaaccca acctgctgga gaatatggtg
     2641 aacaaggaac gcgagcctgc cctgtacgct gggctgaaag cacgactggc agagttcaac
     2701 caggaccccg caaaggcttt cgcaacacct ttttataaac agggaggaca gcaggtgaag
     2761 gcaatccgag tggagcaggt ccagaagagt ggggtgctgg tccgggaaaa taacggagtg
     2821 gctgacaacg catcaatcgt gaggacagat gtcttcatta agaacaacaa attctttctg
     2881 gtgccaatct acacttggca ggtcgctaag ggcatcctgc ccaacaaagc cattgtggct
     2941 cacaagaatg aagacgagtg ggaagagatg gatgagggag ccaagttcaa attttccctg
     3001 tttccaaacg acctggtgga actgaagacc aagaaagagt atttctttgg gtactatatc
     3061 ggactggatc gcgctacagg caatatttca ctgaaagaac atgacgggga gatcagcaag
     3121 gggaaagatg gagtgtaccg ggtgggcgtc aaactggccc tgagcttcga aaagtatcag
     3181 gtggacgagc tggggaagaa caggcagatt tgccggcccc agcagcgaca gcctgtgcgg
     3241 taagaattc
//
