LOCUS       sequence-120816-         801 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S 
            (4Cys to Ser) with N-term Histag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney 
            BJ, Brash AR
  TITLE     On the relationships of substrate orientation, hydrogen abstraction,
            and product stereochemistry in single and double dioxygenations by 
            soybean lipoxygenase-1 and its Ala542Gly mutant.
  JOURNAL   J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.
  PUBMED    16157595
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-11-18"; volume: "280(46)"; pages: "38756-66. 
            Epub 2005 Sep 12"
COMMENT     Sequence Label: pET30Xa-SBL1(160S,NoCys)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..70
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             77..121
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             152..163
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtctggtgcc
       61 acgcggttct ggtatgaaag aaaccgctgc tgctaaattc gaacgccagc acatggacag
      121 cccagatctg ggtaccggtg gtggctccgg tattgagggt cgcatgtttt cagcaggcca
      181 taagatcaaa gggacagtgg tgttgatgcc caagaatgag ttggaagtta accctgatgg
      241 ctcagcagtt gacaacctta atgctttctt gggccgtagt gtctcccttc agctcattag
      301 tgctaccaag gctgatgcac atggaaaagg aaaagttgga aaggatacgt tcttggaagg
      361 gattaatact tcgttaccaa ctttgggagc aggagagtct gcattcaata ttcattttga
      421 atgggacgga agcatgggaa tccccggtgc gttttacata aagaactaca tgcaagttga
      481 gttcttcctc aagagtttga ctcttgaagc catttcaaac caaggaacca tccgctttgt
      541 ttccaactca tgggtttata acactaaact ttacaaaagc gtgcgcattt tctttgccaa
      601 ccatacatat gtacctagtg agacaccagc accacttgtg agttacagag aagaagaatt
      661 gaagagttta agaggaaatg gaacgggaga gcgcaaggaa tatgatagga tctatgatta
      721 tgatgtctac aatgatttgg gcaacccaga taagagtgaa aagttagctc gtccagttct
      781 tggagggtct agcacctttc c
//
