LOCUS       sequence-120853-        1029 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning 
            technology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Grant IM, Balcha D, Hao T, Shen Y, Trivedi P, Patrushev I, Fortriede
            JD, Karpinka JB, Liu L, Zorn AM, Stukenberg PT, Hill DE, Gilchrist 
            MJ
  TITLE     The Xenopus ORFeome: A resource that enables functional genomics.
  JOURNAL   Dev Biol. 2015 Dec 15;408(2):345-57. doi: 
            10.1016/j.ydbio.2015.09.004. Epub 2015 Sep 29.
  PUBMED    26391338
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2015.09.004"; journalName: "Dev Biol"; date: 
            "2015-12-15- 15"; volume: "408"; issue: "2"; pages: "345-57"
COMMENT     Sequence Label: pCSF107mT-GATEWAY-3'-3HA
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(349..367)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(350..367)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtggtacc aagagatctg
      301 aattcttcga aatcgatgga tcctgcaaaa agaacaagta gcttgtattc tatagtgtca
      361 cctaaatcaa gcttgctccg acgtccccag gcagaatggc ggttccctaa acgagcattg
      421 cttatataga cctcccatta ggcacgccta ccgcccattt acgtcaatgg aacgcccatt
      481 tgcgtcattg cccctcccca ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc
      541 atttaccgcc attgacgtca atgggagtac tgccaatgta ccctggcgta cttccaatag
      601 taatgtactt gccaagttac tattaataga tattgatgta ctgccaagtg ggccatttac
      661 cgtcattgac gtcaataggg ggcgtgagaa cggatatgaa tgggcaatga gccatcccat
      721 tgacgtcaat ggtgggtggt cctattgacg tcaatgggca ttgagccagg cgggccattt
      781 accgtaattg acgtcaatgg gggaggcgcc atatacgtca ataggaccgc ccatatgacg
      841 tcaataggaa agaccatata tagagaccat tgacgtcaat gggggagtgg ctatgggcgg
      901 tattaggaag ccccatatat ggtatatggg accgcccatt gggaggggct atctacgtca
      961 ataggaaaac ccatatatgg aatactatat ggcatagggc caatacatag tattgaacct
     1021 ggccaatag
//
