LOCUS       sequence-120895-         952 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a fluorescent chitin reporter under Gal4 control in 
            Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Sobala LF, Wang Y, Adler PN
  TITLE     ChtVis-Tomato, a genetic reporter for in vivo visualization of 
            chitin deposition in Drosophila.
  JOURNAL   Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. 
            Epub 2015 Sep 22.
  PUBMED    26395478
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.126987"; journalName: "Development"; date: "2015-11-15-
            15"; volume: "142"; issue: "22"; pages: "3974-81"
COMMENT     Sequence Label: pWalium-ChtVis-Tomato
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(668..823)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(913..936)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
       61 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc ccgcggctct agttctttgc
      121 aatctgtaag cataagcaaa gaaaaaatgg gttagctaat gagttttata actgcgtatt
      181 atttgaaaat atcaaaaatg tatacaaaaa catattttca atattttcaa agtgtatttt
      241 taggggttgt taatcgtgtg tgatgcctac ctgatgccaa caattgtcta gcagatcaga
      301 actagtttgc tctagattag ttcacatagg ccatgcccgc tcccttgcga tcgaagtgac
      361 gcacgcggac gaagaggaag gcggcgacta gagaggcgac ggccaggacg agcagcagca
      421 tcaccaatcc gcccacaaag ctgggcaccg acatgcagat ggtctccgca tccgcggacc
      481 tggccgactg gatgaccacc gtctcgttgc tggcattgct gttcagcgca aagttaacgt
      541 ctccgggcgc caccacctgg ataattcggc tggtgttcac atcggtcatc tcctgcgcgt
      601 tcctcttgta gatcctctgt ggcttcacca caatgtccat ggtgtctctg cgccttctcg
      661 aggcaccttg aagctgccac aaggcaggaa cgttggatgg ttcccatcct gccaaggagg
      721 tgtggggctg caaacattta tacgtcttgc cgttatatgt gaccaattgt cccgcagtat
      781 aagctgtgtt gacctgccaa gcggatacac caggatttgt cgtatagatc ttgtacagct
      841 cgtccatgcc gtacaggaac aggtggtggc ggccctcgga gcgctcgtac tgttccacga
      901 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt gtccacgtag ta
//
