LOCUS       sequence-120962-         781 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  luciferase reporter for hA33 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Johnstone CN, White SJ, Tebbutt NC, Clay FJ, Ernst M, Biggs WH, 
            Viars CS, Czekay S, Arden KC, Heath JK
  TITLE     Analysis of the regulation of the A33 antigen gene reveals 
            intestine-specific mechanisms of gene expression.
  JOURNAL   J Biol Chem. 2002 Sep 13;277(37):34531-9. Epub 2002 Jul 11.
  PUBMED    12114523
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-09-13"; volume: "277(37)"; pages: "34531-9. Epub
            2002 Jul 11"
COMMENT     Sequence Label: 0.41 hA33 luciferase
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    475..566
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(507..526)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    580..628
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt accggaatgc
       61 caagcttact tagatcgcag atctcgagca ctggcagcct ccatacaggt ctgagctgtc
      121 tggttaaagc tacagcagct tctggcctgg cccaactgga ggtccctgca attttccctc
      181 ttctcaccca cctctgccca gcctcagctg gattggcagg tgagctcctc agctggattg
      241 gcaggtgagc tggggctggg ctcatgtgtc acctactcca aaggtcccga gaggagtctt
      301 ccctacttcc ctcaagttcc ccttaaacca gccacctccc ctgccttctc cctacttagc
      361 aggtgtacaa agtcctagac tgctcacaaa ggggtaggcc tctcttataa ctgacaggaa
      421 ataaataata aaaagggaaa tgcactcaga ggtagggtac cggtacctat cgatagagaa
      481 atgttctggc acctgcactt gcactgggga cagcctattt tgctagtttg ttttgtttcg
      541 ttttgttttg atggagagcg tatgttagta ctatcgattc acacaaaaaa ccaacacaca
      601 gatgtaatga aaataaagat attttattgc ggccgctcca agtacctccc gtaccttaat
      661 attacttact tatcatggta gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg
      721 cccttcccaa cagttgcgca gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt
      781 g
//
