LOCUS       sequence-120986-       10424 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            Lentivirus production).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pLenti-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..10424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4012..4129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(4135..4155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4140..4469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4320..4455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4479..5078
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4479..4498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4975..4995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        5150..5453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5454..5657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5608..5628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5654..5678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5757..5774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5784..5813
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     7178..7199
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(7257..7259,7260..7262,7263..7775)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(7302..7323)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7563..7582)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             8427..8444
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    8486..9074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8539..8559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8957..8968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9165..9398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9470..9591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9507..9526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9561..9580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9610..9745
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9777..9796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9778..9796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9806..9823)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9806..9822)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9815..9837)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9967..10422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10054..10073)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10264..10285
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacctga aagcgaaagg gaaaccagag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcctcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta ttgtggaatg
     4141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg
     4501 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4561 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     4981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc
     5041 gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     5101 gcccgaccga aaggagcgca cgacccaatc gataagcttg ggagttccgc gttacataac
     5161 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     5221 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     5281 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     5341 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     5401 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     5461 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     5521 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     5581 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     5641 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     5701 gttttgacct ccatagaaga caccgactct agaggatccg gagatatacc atggcacacc
     5761 atcaccacca tcacgggagc ggggagcaga aactaatatc agaggaggat cttggctcag
     5821 gatcctccac cgacggcatg gacgagctgt acaagggtgc tagcggagga atggtgagca
     5881 agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg
     5941 agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg
     6001 acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg
     6061 acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac cccgacgaca
     6121 tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct
     6181 tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
     6241 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
     6301 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
     6361 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
     6421 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
     6481 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
     6541 cccgcaactc cggagacatc cagaaccctg acatcacgag ttcacgatac cgtgggctcc
     6601 cagcacctgg acctagccca gctgataaga aacgctcagg gaagaagaag atcagcaaag
     6661 ctgatattgg tgcacccagt ggattcaagc atgtcagcca cgtggggtgg gacccccaga
     6721 atggatttga cgtgaacaac ctcgaccccg acctgcgcag tctgttctcc agggcaggaa
     6781 tcagcgaggc ccagctcacc gacgccgaga cctctaaact tatctacgac ttcattgagg
     6841 accagggtgg gctggaggct gtgcggcagg agatgaggcg ccaggagcca cttccgcccg
     6901 ggacttctgg ttctggtaaa cctggttctg gtgaaggttc tactaaaggg acttctggtt
     6961 ctggtaaacc tggttctggt gaaggttcta ctaaagggac ttctggttct ggtaaacctg
     7021 gttctggtga aggttctact aaagggactt ctggttctgg taaacctggt tctggtgaag
     7081 gttctactaa aggggcggcc gccatgctgc tgcccgacaa ccactacctg agctaccagt
     7141 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7201 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggtggcagc ggtggcatgg
     7261 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     7321 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     7381 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     7441 tgaccaccct gggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc
     7501 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     7561 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     7621 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     7681 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     7741 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     7801 actaccagca gaacaccccc atcggcgacg gccccgtggg taccatgcag acaattaagt
     7861 gtgttgttgt tggtgatggt gctgttggta aaacatgtct cctgatatcc tacacaacaa
     7921 acaaattccc atcggaatat gtaccaactg tttttgacaa ctatgcagtc acagttatga
     7981 ttggtggaga gccatacact cttggacttt ttgatactgc agggcaagag gattatgaca
     8041 gactacgacc gctaagttat ccacagacag atgtttttct agtatgtttc tcagtggtct
     8101 ctccatcctc atttgaaaat gtgaaagaaa agtgggtgcc tgagataact caccactgtc
     8161 caaagactcc tttcttgctt gttgggaccc aaattgatct cagagatgac ccctctacta
     8221 ttgagaaact tgccaagaac aaacagaagc ctattactcc agagactgct gaaaagctgg
     8281 cgcgggatct gaaggctgtc aagtatgtgg agtgctctgc cctcacacag aaaggcctaa
     8341 agaatgtgtt tgatgaagca atattggctg ccctggagcc tccagaaccg aagaagagcc
     8401 gcaggtgtgt gctgctatga ctcgagcacc accatcacca tcaccaggat cctgcagtct
     8461 agagctagca tatgaattcg tcgacaatca acctctggat tacaaaattt gtgaaagatt
     8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9001 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9061 ggccgcctcc ccgcctggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     9121 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     9181 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     9241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     9421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     9481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9601 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9721 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct
     9781 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9961 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    10021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    10381 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcc
//
