LOCUS       sequence-121276-         863 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  C-terminal fusion to moxGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: moxGFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(105..107,108..110,111..824)
                     /codon_start=1
                     /product="monomeric GFP variant optimized for use in 
                     oxidizing environments such as the secretory pathway 
                     (Costantini et al., 2015)"
                     /label=moxGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQSFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(150..171)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg tccaagggcg
      121 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      181 acaagttctc cgtgcggggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
      241 agttcatcag caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      301 cctacggcgt gcagagcttc tcccgctacc ccgaccacat gaagcgccac gacttcttca
      361 agagcgccat gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca
      421 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      481 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      541 tcaactccca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
      601 tcaagatccg ccacaacgtg gaggacggct ccgtgcagct cgccgaccac taccagcaga
      661 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg tccacccagt
      721 ccaagctgtc caaagacccc aacgagaagc gcgatcacat ggtccttctg gaattcgtga
      781 ccgccgccgg gatcactcac ggcatggacg agctgtacaa gtaagcggcc gcgactctag
      841 atcataatca gccataccac att
//
