LOCUS       sequence-121598-        3045 bp    DNA     circular SYN 14-APR-2025
DEFINITION  miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for 
            targeting to the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y
  TITLE     Photo-inducible cell ablation in Caenorhabditis elegans using the 
            genetically encoded singlet oxygen generating protein miniSOG.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 
            10.1073/pnas.1204096109. Epub 2012 Apr 24.
  PUBMED    22532663
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1204096109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-05-8- 8"; volume: "109"; issue: "19"; pages: "7499-504"
COMMENT     Sequence Label: pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             189..503
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
     protein_bind    complement(519..618)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(635..654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(636..654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(659..675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(777..796)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             788..1597
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(855..874)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1744..2332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2233..2252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2486..2503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2662..2689)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2781..2867)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2916..2938
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2930..2947
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2931..2947
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(2963..3045,1..17)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtacaaaaaa gcaggctatg tcggacacaa ttcttggttt caacaaatca aacgtcgttt
       61 tggctgctgg aattgctgga gccgctttcc tcggctactg catttacttc gatcataaga
      121 gaatcaacgc tccagactac aaggacaaga ttaggcaaaa gagacgtgcc caggctggag
      181 catccggaga gaaaagtttc gtgataactg atccacggct gccagacaat cccatcatct
      241 tcgcatccga tggcttcctg gagctgaccg agtattccag agaggagatc ctgggccgca
      301 atggccgctt tctgcaggga ccagagacag accaggccac agtgcagaag attcgcgatg
      361 ccattagaga tcagcgcgag attaccgtgc agctgataaa ctacacaaaa agcgggaaga
      421 aattctggaa cctcctgcac ctccagccca tgagggacca gaagggtgag ctccagtatt
      481 tcatcggagt gcagctggat ggaggatctt ccggataaac ccagctttct tgtacaaagt
      541 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
      601 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
      661 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
      721 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
      781 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
      841 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
      901 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
      961 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     1021 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     1081 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     1141 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     1201 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     1261 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     1321 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     1381 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     1441 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     1501 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     1561 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     1621 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     1681 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     2581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
     2641 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
     2701 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
     2761 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     2821 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
     2881 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
     2941 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
     3001 gttgcaacaa attgatgagc aatgcttttt tataatgcca acttt
//
