LOCUS       sequence-121771-         418 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo
FEATURES             Location/Qualifiers
     source          1..418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..68)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gttagcttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
       61 ggaacttcaa gatcccctga ttccctttgt caacagcaat ggatttatct ctgatgttac
      121 attgcacaag ataaaaatat atcatcatga tcagtcctgc tcctcggcca cgaagtgcac
      181 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
      241 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
      301 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
      361 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcg
//
