LOCUS       sequence-121905-        3072 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Reporter Plasmid - deGFP driven by a qacR responsive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES001 (pQacA-deGFP)
FEATURES             Location/Qualifiers
     source          1..3072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..780
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(187..206)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      801..895
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1319..1336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1453..1998)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1533..1552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(2270..2291)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2619..2638
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2878..2899
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             2936..2958
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatggatgtg agaccttgat cgggcacgta agaggttcca actttcacca taatgaaata
       61 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
      121 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      181 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      241 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      301 cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt
      361 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      421 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      481 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      541 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      601 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
      661 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      721 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
      781 tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      841 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      901 cctcggtgag aatccaagcc tcgatcaacg tctcattttc gccaaaagtt ggcccagggc
      961 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     1021 ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg
     1081 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     1141 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcggt agcggagtgt
     1201 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     1261 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     1321 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     1381 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     1441 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     1501 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     1561 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1621 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1681 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     1741 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     1801 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     1861 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     1921 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     1981 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca
     2041 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
     2101 ttctcatgtt tgacagctta tcatcgataa gcttccgatg gcgcgccgag aggctttaca
     2161 ctttatgctt ccggcctctc aagggcatcg gtcgacacag aaaagcccgc ctttcggcgg
     2221 gctttgctcg agttattcac cagatgcttc attagatccc ggcggcggtc acgaactcca
     2281 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     2341 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     2401 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     2461 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     2521 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     2581 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     2641 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     2701 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     2761 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     2821 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     2881 cgccgtccag ctcgaccagg atgggaacaa cgccagtgaa aagctccatg gtatatctcc
     2941 ttcttaaagt taaacaaaat tattgctttt aagttccctc caatccttat agaccgtgcg
     3001 atcggtctat aaggattata atctattttt ttacatatta caacaaaaaa tcttgcgaaa
     3061 tttaaatgat gc
//
