LOCUS       sequence-122591-         779 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-STRDHFR
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             67..90
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttggaattcg ctagctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
       61 gcaagctgga gccacccgca gttcgagaaa ggatccatca gtctgattgc ggcgctagcg
      121 gtagatcgcg ttatcggcat ggaaaacgcc atgccatgga acctgcctgc cgatctcgcc
      181 tggtttaaac gcaacacctt aaataaaccc gtgattatgg ggcgccatac ctgggaatca
      241 atcggtaggc ctttgcccgg ccgcaaaaat attatcctca gcagtcaacc cgggaccgat
      301 gatcgggtta cctgggttaa atcggtcgac gaagccatcg cggcgtgtgg tgacgtacca
      361 gaaatcatgg tgattggcgg cggacgcgtt tatgaacagt tcttgccaaa agcgcaaaaa
      421 ctgtatctga cgcatatcga tgcagaagtg gaaggcgaca cccattttcc ggattacgag
      481 ccggatgact gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactcgcat
      541 agctattgtt tcgaaatcct cgagcgtcgt gccggctaat gtacaagatc cagatctaag
      601 cttggtacca cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atcgggatta gcccgcctaa tgagcgggc
//
