LOCUS       sequence-122657-         920 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Neisseria meningitidis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX405 Neisseria meningitidis Cas9
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        84..193
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    195..219
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             248..274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             278..298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tggatcttaa tacgactcac tataggggaa
       61 ttgtgagcgg ataacaattc cgggaccttt aattcaaccc aacacaatat attatagtta
      121 aataagaatt attatcaaat catttgtata ttaattaaaa tactatactg taaattacat
      181 tttatttaca atcaacaagt ttgtacaaaa aagcaggcta aggagatata ccaccggtgc
      241 caccatgtac ccatacgatg ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga
      301 agcgtccgct gccttcaaac ctaaccctat caactacatc ctggggctgg acattggaat
      361 cgcttcagtg ggctgggcta tggtggaaat cgacgaggat gaaaacccta tttgcctgat
      421 cgacctgggg gtcagagtgt ttgagagggc tgaagtgcca aagacaggag atagtctggc
      481 tatggcacgg agactggcaa ggtcagtgag gcgcctgact cgacggagag cacacaggct
      541 gctgcgagct aggcgcctgc tgaagcgaga gggagtgctg caggccgctg acttcgatga
      601 aaacgggctg atcaagagcc tgcccaatac cccttggcag ctgagagcag ccgctctgga
      661 caggaagctg acaccactgg agtggtctgc cgtgctgctg cacctgatca agcatcgcgg
      721 atacctgagt cagcgaaaaa acgagggcga aacagcagat aaggagctgg gagccctgct
      781 gaaaggcgtg gctgacaatg cccacgctct gcagaccggc gattttagga cacccgcaga
      841 actggccctg aacaagttcg agaaagaatc cgggcacatt cgaaatcagc ggggagacta
      901 ttctcatacc ttcagtcgca
//
