LOCUS       sequence-122832-         807 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  The prostate specific promoter controls expression of FlpO linked to
            a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, 
            and SMAD4 ex2 and ex 9 are expressed by the U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2,...
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(476..551)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(630..649)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tctggacctc actgacaagg tgcagagtag cttcctgctt tttatagctg cttttcagga
       61 tgattggcat gggcagtgtc ttgcatccca gttggcagtt gtacacagac attgacattg
      121 tgtcatctag tatacaaata ggttcttgga gtactttact aggcatggac aatgcccaat
      181 gcctgtccca ttcttcaggc atatttttat ttgtgggctt tatgtcccta ttaagaaaaa
      241 gactaagaac aagatgctat catattttct taactggaat ggtagatgtt taaacatgat
      301 gactattaca ttgtgtcatc tagtatacaa ataggttctt ggagtacttt actaggcatg
      361 gacaatgccc aatgcctgtc ccattcttca ggcatatttt tatttgtggg ctttatgtcc
      421 ctattaagaa aaagactaag aacaagatgc tatcataact agtctcgaga aaaaagcacc
      481 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
      541 agctctaaaa cgtggacatt ggagagttga cggtgtttcg tcctttccac aagatatata
      601 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      661 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      721 ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca gccttgtatc
      781 gtatatgcaa atatgaagga atcatgg
//
