LOCUS       sequence-122983-         903 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into
            a MIN-tagged locus using Neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-GFP-Dnmt3b1-Poly(A)-NeoR
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..69
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    240..287
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     312..331
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(630..649)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacg gatgatcctg acgacggaga ccgccgtcgt
       61 cgacaagccg ggatctcgat gtaagcttgc cgccccgacg ttggctgcga gccctgggcc
      121 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
      181 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
      241 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
      301 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
      361 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
      421 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
      481 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
      541 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
      601 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
      661 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
      721 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
      781 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
      841 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
      901 ttc
//
