LOCUS       sequence-122999-         848 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Dominant negative Dynamin 1 mutant tagged with mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: Dyn1(K44A)-mRFP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..803)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(126..803)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(188..211)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(386..405)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     655..673
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgctttagg cgccggtgga gtggcggccc tcggcgcgct cgtactgttc cacgatggtg
      181 tagtcctcgt tgtgggaggt gatgtccagc ttgatgtcgg tcttgtaggc gccgggcagc
      241 tgcacgggct tcttggccat gtaggtggtc ttgacctcgg cgtcgtagtg gccgccgtcc
      301 ttcagcttca gcctcatctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      361 tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
      421 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
      481 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
      541 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
      601 ccgtactgga actgagggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc
      661 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
      721 tcgaactcgt ggccgttcac ggagccctcc atgcgcacct tgaagcgcat gaactccttg
      781 atgacgtcct cggaggaggc catggtggcg accggtggat cccgggcccg cggtaccgtc
      841 gactgcaa
//
