LOCUS       sequence-123011-         772 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11055
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(110..455)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(147..167)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atccatcttg ttcaatcatt gtatcgataa ttgtaaatgt aattgtaatg ttgtttgttg
       61 tttgttgttg ttggtaattg ttgtaaaaat gagctcttat actcgagcgt gtcctctcca
      121 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
      181 catcccttac gtcagtggag atgtcacatc aatccacttg ctttgtagac gtggttggaa
      241 cctcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
      301 cagagagatc ttgaatgata gcctttcctt tatcgcaatg atggcatttg taggagccac
      361 cttccttttc tactgtcctt tcgatgaagt gacagatagc tgggcaatgg aatccgagga
      421 ggtttcccga aattatcctt tgttgaaaag tctcaatagc cctttgatct tctgagactg
      481 tatctttgac atttttggag tagaccagag tgtcgtgctc caccatgtta tcacatcaat
      541 ccacttgctt tgtagacgtg gttggaacct cttctttttc cacgatgctc ctcgtgggtg
      601 ggggtccatc tttgggacca ctgtcggcag agagatcttg aatgatagcc tttcctttat
      661 cgcaatgatg gcatttgtag gagccacctt ccttttctac tgtcctttcg atgaagtgac
      721 agatagctgg gcaatggaat ccgaggaggt ttcccgaaat tatcctttgt tg
//
