LOCUS       sequence-12315-e        8565 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag
            J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A, 
            Anderson DJ
  TITLE     Genetic dissection of an amygdala microcircuit that gates 
            conditioned fear.
  JOURNAL   Nature. 2010 Nov 11. 468(7321):270-6.
  PUBMED    21068836
REFERENCE   2  (bases 1 to 8565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Nov 11. 468(7321):270-6."
COMMENT     Sequence Label: pAAV-EF1a-FLEX-GTB
FEATURES             Location/Qualifiers
     source          1..8565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        242..1419
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          473..1410
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1367..1387
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1441..1450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1453..1486
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1549..1582
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3173..3232)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(3722..3775)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(3791..4501,4502..4504))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(3791..4501,4502..4504))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3833..3854)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4182..4201
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4441..4462
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(4513..4546)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4617..4650)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4692..5280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4745..4765)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5163..5174)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5312..5788
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5511..5530)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5828..5968
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5828..5957
                     /label=AAV2 ITR
     rep_origin      6043..6498
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6130..6149)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6340..6361
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6515..6534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6634..6656
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6694..6712)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6780..6884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6885..6953,6954..7745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7103..7122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7916..8504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8405..8424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagcc
     1441 gccaccatgg ccataacttc gtataaagta tcctatacga agttatatca aaataggaag
     1501 accaatgctt caccatcgac ccgaattgcc aagcatcacc atcgacccat aacttcgtat
     1561 aatgtatgct atacgaagtt atacttcaac tagttcacag tctggtctcg ccgccgctct
     1621 tgtggctctc ccagctgctg atgatcttgc cgctctgggg ggtcacgctc acctctctgc
     1681 cggtgcctct caggttgtgc tgggtgggct cgcttctgtt cactcttctg cagcaggtca
     1741 tcaggaagat gatcagcatc agggcggtca gggcgccggc gctcagcagc acgtacttgc
     1801 cccagttggg caggcccagg tccacgccgc tcacctggtt gtgcacgtcg ggcaggtgca
     1861 cctccacgaa gtcctcggcc tcgtcgccgt ccttgaacac ggtgctgggg tcggccaggg
     1921 ggtgcaccag ggggatcacg ctgctctcca gcagctccat gtgctgctgc agcaggctgc
     1981 tctgcatctc ggggatcagc acgttgccgt cggggcccag gatgatgccg ttgaagaaca
     2041 cgccgttcac gtgggggtgg catctgccgc ccactctcag gcagcccttg ctgggcagga
     2101 tctcgttcca ggttctcacg ctcttgtagt gggcgtcggc ctccatcagg gtcttgttga
     2161 agatggtgta ggccttgccg aagccgggca ccagctttct caggtggctc agtcttctga
     2221 agctcacgct cttggtggtc atgatgctct ccagggcgtc caggcactcc tctctctttc
     2281 tcaccagctc ctccaccacc aggtgctcga tctcgtcgct tctgaagtcg tgcaggttca
     2341 ccagcttgtc gggggggcac cacttggtct cgttgctggt ctgcatgctc acccaggtgc
     2401 cgtccatcag tctcaggccc agcacgccgc acagcttcag cttgcaggcg cccttcaggc
     2461 tcttgtacag gcctctctcg tccacgaagc cgcaggtctc gctgcccttg ctggctctct
     2521 tgcctctgct gttggtgaag atgtcgcagc tcatgcccag tctggggttc tcgggcatcc
     2581 agatggtgta gtcgtggttg gtgctgcagt aggtgctgct cacggccacg ccgctgcact
     2641 tgccgctggg gaacactctg ctgtgcaggc ttctgtcgta ggggtccagg tcggccacgc
     2701 tggggctgat gatcaccagg ctctccttgg tggtcttcac ggttctcagc catctgtagt
     2761 cggggtaggg gttgtgcagg ctctcctcgt atctggggtc gccggccatc ttccagttgt
     2821 aggcggctct gcaggcgtcg ggggtgggtc tgaagtgctt tctcttgaag gtggtggtca
     2881 cgtagcccac gaagttggtg taggtctcgg cctcggtcac cacgccggtg caggtgaagc
     2941 cgttcacctt gatggccagg atgtagccca ccttcagctc catgtagctg aagccgctca
     3001 ggttggtgca gccctcgtcc tccaccacca ggttgttggg gcagctcagg tggtggatgt
     3061 cgatggggct ccaggggccc agcttgtcgg ggatggtgta gatggggaac ttgccgaagc
     3121 acagggggaa caccagcagg ggcacgaaca gcagggcctg gggcactccg gaaggaccgg
     3181 ggttactttc aacatcgcca gcgagtttca acaaagcgta gttagtacat tgaccactac
     3241 cgctagcgga gaacaagtct gcctggctct tgtcaggcac cagcagctcc ttggatgcgc
     3301 tttcaagact gaagatgtca gacctgcttt ttgctttgga cttcccccat gcagcgatac
     3361 cacccacagc taccaggcag cacaggagca ctgcagtgat cagcatccac atgcggccgt
     3421 gattcctggc tggcagagca cgtccaggag caggggcagt gggagcctct gtgccgttgt
     3481 ccgtgggcac cgcggggatc gcgctggtcc cgcagcccca ctcgtcccgc ccgtcgtcgc
     3541 agtcggggtg tccgtcgcac agccagtcct gcgggtaaca ctccccgtgg gcaccgggcg
     3601 gctccgagca gcggaactga ccgggggggc aacgggacaa agaaccgtta ccggacccgt
     3661 taccggtcac gttaccgggc agcagcagca gcagcagcgc gggcagcagc cgcgcatcga
     3721 ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg ccctctccac
     3781 taccaagctt cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     3841 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     3901 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3961 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4021 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4081 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4141 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4201 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4261 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4321 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4381 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4441 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4501 tcacctcgac gcataacttc gtataggata ctttatacga agttatcttt gggattcttc
     4561 ctattttgat ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa
     4621 cttcgtatag catacattat acgaagttat gtccctcgaa gaggttcgaa ttcgatatca
     4681 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4741 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4801 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4861 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4921 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4981 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5041 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5101 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5161 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5221 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5281 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     5341 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     5401 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     5461 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     5521 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     5581 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     5641 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     5701 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     5761 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     5821 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     5881 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     5941 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     6001 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     6061 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     6121 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6181 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6241 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6301 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6361 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     6421 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6481 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8041 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
