LOCUS       sequence-123257-         840 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a fluorescent chitin reporter under Gal4 control in 
            Drosophila..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Sobala LF, Wang Y, Adler PN
  TITLE     ChtVis-Tomato, a genetic reporter for in vivo visualization of 
            chitin deposition in Drosophila.
  JOURNAL   Development. 2015 Nov 15;142(22):3974-81. doi: 10.1242/dev.126987. 
            Epub 2015 Sep 22.
  PUBMED    26395478
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.126987"; journalName: "Development"; date: "2015-11-15-
            15"; volume: "142"; issue: "22"; pages: "3974-81"
COMMENT     Sequence Label: pWalium-ChtVis-Tomato
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..144
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(157..190)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    206..300
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(313..346)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    364..794
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 acgaagcgcc tctatttata ctccggcgct cgctgtcgac gagtctccgc tcggaggaca
       61 gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg gaggacagta
      121 ctccgctcgg aggacagtac tccgacctgc ggatccataa cttcgtatag catacattat
      181 acgaagttat ccatgggagt ctccgctcgg aggacagtac tccgctcgga ggacagtact
      241 ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga cagtactccg
      301 acctgcctgc agataacttc gtatagcata cattatacga agttataagc tggtactact
      361 agtgttgttg gttggcacac cacaaatata ctgttgccga gcacaattga tcggctaaat
      421 ggtatggcaa gaaaaggtat gcaatataat aatcttttat tgggtatgca acgaaaattt
      481 gtttcgtcaa cgtatgcaat attctttatt aaaagagggt atgcaatgta ttttattaaa
      541 aacgggtatg caatataata atcttttatt gggtatgcaa cgaaaatttg tttcgtcaaa
      601 gtatgcaata ttttttatta aaagagggta tgcaatgtat tttattaaaa acgggtatgc
      661 aataaaaaat tatttggttt ctctaaaaag tatgcagcac ttattttttg ataaggtatg
      721 caacaaaatt ttactttgcc gaaaatatgc aatgtttttg cgaataaatt caacgcacac
      781 ttattacgtg gccaactagt gtaccgaatt aggccttcta gtggatcaat tcggcttgtc
//
